Autophosphorylation of Entamoeba histolytica atypical kinase EhAK1

ABSTRACT: Phagocytosis plays a key role in survival and pathogenicity of Entamoeba histolytica. We have recently demonstrated that an atypical kinase EhAK1 is involved in phagocytosis in this parasite. It is recruited to the phagocytic cups through interaction with EhCaBP1. EhAK1 manipulates actin dynamics by multiple mechanisms including phosphorylation of G-actin. Biochemical analysis showed that EhAK1 is a serine/threonine kinase with broad ion specificity and undergoes multiple trans-autophosphorylation. Multiple autophosphorylation sites were identified by mass spectrometry. Out of these Thr279 appears to be involved in both autophosphorylation as well as substrate phosphorylation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Entamoeba Histolytica Hm-1:imss-a

SUBMITTER: gagan jhingan  

LAB HEAD: ALOK BHATTACHARYA

PROVIDER: PXD002691 | Pride | 2018-10-24

REPOSITORIES: Pride