Proteomics

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Phosphoproteomic analysis of liver of Dicer knockout mice

ABSTRACT: Dicer is an essential ribonuclease involved in the biogenesis of miRNA. Previous studies have reported that Dicer is dysregulated in multiple types of cancers. To investigate the downstream phosphoproteome of Dicer, we carried out phosphotyrosine profiling of the liver of Dicer knockout of mice. We employed antibody-based enrichment of phosphotyrosine containing peptides coupled with spike-in SILAC-based quantitation. Over 400 phosphoisites were identified in each condition while 290 phosphosites were quantitated in common. Amongst these, several key signaling molecules like receptor tyrosine kinase MET, MET, PDGFR, EPHA2, EPHB4 and IGF1R/INSR were hyperphosphorylated. Also, non-receptor tyrosine kinases of Src family and their downstream effector signaling partners including IRS2 and STAT3were found to be hyperphosphorylated. Our study indicates that there is significant alteration in the signaling pathways upon ablation of Dicer.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD000927 | Pride | 2016-07-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Dicer_Control_1.RAW Raw
Dicer_Control_1_pY.msf Msf
Dicer_Control_1_pY.pep.xml Pepxml
Dicer_Control_2.RAW Raw
Dicer_Control_2_pY.msf Msf
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Publications

Ablation of Dicer leads to widespread perturbation of signaling pathways.

Sahasrabuddhe Nandini A NA   Huang Tai-Chung TC   Kumar Praveen P   Yang Yi Y   Ghosh Bidyut B   Leach Steven D SD   Chaerkady Raghothama R   Pandey Akhilesh A  

Biochemical and biophysical research communications 20150530 3

Dicer is an essential ribonuclease involved in the biogenesis of miRNAs. Previous studies have reported downregulation of Dicer in multiple cancers including hepatocellular carcinoma. To identify signaling pathways that are altered upon Dicer depletion, we carried out quantitative phosphotyrosine profiling of liver tissue from Dicer knockout mice. We employed antibody-based enrichment of phosphotyrosine containing peptides coupled with SILAC spike-in approach for quantitation. High resolution ma  ...[more]

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