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Proteomic analyses of embryogenic tissues in Picea balfouriana treated by 6-BAP

ABSTRACT: In this study, iTRAQ technology was applied to characterize the genes and proteins that are differentially expressed among tissues under the influence of different levels of 6-BAP(2.5 μM, 3.6 μM and 5 μM). There were two biological and three technological replicates for each treatment. Two biological replicates from the 2.5 μM treatment were labeled with reagents 115 and 121, while iTRAQ tags 117 and 119 were used to label two biological replicates from the 3.6 μM treatment, and two biological replicates from the 5 μM treatment were labeled with reagents 116 and 118.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Picea Likiangensis Var. Rubescens

TISSUE(S): Cell Culture

SUBMITTER: Qingfen Li

LAB HEAD: Junhui Wang

PROVIDER: PXD001022 | Pride | 2014-09-23

REPOSITORIES: Pride

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			Action	DRS
	yunshan-1-1.mgf	Mgf
	yunshan-1-1.wiff	Wiff
	yunshan-1-1.wiff.scan	Wiff
	yunshan-1-2.mgf	Mgf
	yunshan-1-2.wiff	Wiff

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Publications

Transcriptomic and proteomic analyses of embryogenic tissues in Picea balfouriana treated with 6-benzylaminopurine.

Li Qingfen Q Zhang Shougong S Wang Junhui J

Physiologia plantarum 20141021 1

The cytokinin 6-benzylaminopurine (6-BAP) influences the embryogenic capacity of the tissues of Picea balfouriana during long subculture (after 3 months). Tissues that proliferate in 3.6 and 5 µM 6-BAP exhibit the highest and lowest embryogenic capacity, respectively, generating 113 ± 6 and 23 ± 3 mature embryos per 100 mg of tissue. In this study, a comparative transcriptomic and proteomic approach was applied to characterize the genes and proteins that are differentially expressed among tissue ...[more]

PMID: 25200684

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Expression data from Butyroside D treated B16F10 cells [2uM]

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Candidate proteins involved in the calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis

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