Dataset Information

Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol

ABSTRACT: Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) <20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Neuroblastoma

SUBMITTER: JIMMY RODRIGUEZ

LAB HEAD: Magno Junqueira

PROVIDER: PXD010776 | Pride | 2018-08-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Rodriguezetal2018iTRAQnoteTablesS1-S6.xlsx	Xlsx
	VL_0001676_HILIC14NMR1.raw	Raw
	VL_0001677_HILIC14NMR2.raw	Raw
	VL_0001678_HILIC14NMR3.raw	Raw
	VL_0001683_HILIC15NMR1.raw	Raw

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Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol.

Murillo Jimmy Rodriguez JR Pla Indira I Goto-Silva Livia L Nogueira Fábio C S FCS Domont Gilberto B GB Perez-Riverol Yasset Y Sánchez Aniel A Junqueira Magno M

Analytical biochemistry 20180823

Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) < 20% between biological replicates and the v ...[more]

PMID: 30145218

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