Dataset Information

Redox Proteome of Skeletal Muscle

ABSTRACT: Adult and aged mouse gastrocnemius muscle analysed by redox shotgun proteomics, applying a label free quantitative proteomic approach that includes a differential Cysteine labelling step allowing simultaneous identification of up and down regulated proteins between samples and also the identification and relative quantification of the reversible oxidation state of susceptible redox Cysteine residues.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle Cell, Gastrocnemius

SUBMITTER: Brian McDonagh

LAB HEAD: Brian McDonagh

PROVIDER: PXD001054 | Pride | 2014-09-04

REPOSITORIES: Pride

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Publications

Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging.

McDonagh Brian B Sakellariou Giorgos K GK Smith Neil T NT Brownridge Philip P Jackson Malcolm J MJ

Journal of proteome research 20140916 11

The molecular mechanisms underlying skeletal muscle aging and associated sarcopenia have been linked to an altered oxidative status of redox-sensitive proteins. Reactive oxygen and reactive nitrogen species (ROS/RNS) generated by contracting skeletal muscle are necessary for optimal protein function, signaling, and adaptation. To investigate the redox proteome of aging gastrocnemius muscles from adult and old male mice, we developed a label-free quantitative proteomic approach that includes a di ...[more]

PMID: 25181601

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