Proteomics

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Time-course of ischemia-reperfusion in a pig model

ABSTRACT: We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the pig ischemic heart tissue during the first 24h after I/R. We used 40 castrated male Large-White pigs weighing 30 to 40 kg. Closed-chest 40 min I/R was performed in 36 animals and they were sacrificed at 20 min, 40 min, 80 minutes, 2 hours, 6 hours, 12 hours and 24 hours after reperfusion after reperfusion (n = 4 per group). 4 animals were sacrificed with no intervention other than baseline cardiac magnetic resonance ( CMR), and served as controls. A new group of 4 animals were sacrificed at 120 minutes after artery occlusion without reperfusion, and other group of 4 animals were subjected to ischemic preconditioning prior to I/R and sacrificed at 24h. Myocardial tissue samples from ischemic area were collected for proteomics analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Heart

SUBMITTER: Inmaculada Jorge  

LAB HEAD: Jesús Vázquez Cobos

PROVIDER: PXD045771 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

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Action DRS
PIG_OXI_TMT1_CE27_FrAll.msf Msf
PIG_OXI_TMT1_CE27_FrAll.raw Raw
PIG_OXI_TMT1_CE27_FrAll.txt Txt
PIG_OXI_TMT1_CE30_Fr1.msf Msf
PIG_OXI_TMT1_CE30_Fr1.raw Raw
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Publications

While reperfusion, or restoration of coronary blood flow in acute myocardial infarction, is a requisite for myocardial salvage, it can paradoxically induce a specific damage known as ischemia/reperfusion (I/R) injury. Our understanding of the precise pathophysiological molecular alterations leading to I/R remains limited. In this study, we conducted a comprehensive and unbiased time-course analysis of post-translational modifications (PTMs) in the post-reperfused myocardium of two different anim  ...[more]

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