Dataset Information

Proteomic analysis of cAMP-mediated signaling during differentiation of 3T3-L1 preadipocytes

ABSTRACT: Initiation of adipocyte differentiation is promoted by the synergistic action of insulin/insulin-like growth factor, glucocorticoids, and agents activating cAMP-dependent signaling. The action of cAMP is mediated via PKA and Epac, where at least part of the PKA function relates to strong repression of Rho kinase activity, whereas Epac counteracts the reduction in insulin/insulin-like growth factor signaling associated with complete repression of Rho kinase activity. However, detailed knowledge of the Epac-dependent branch and the interplay with PKA is still limited. In the present study, we present a comprehensive evaluation of Epac-mediated processes and their interplay with PKA during the initiation of 3T3-L1 preadipocyte differentiation using a combination of proteomics, molecular approaches and bioinformatics. Proteomic analyses revealed 7 proteins specifically regulated in response to Epac activation, 4 in response to PKA activation, and 11 in response to the combined activation by Epac and PKA during the initial phase of differentiation. Network analyses indicated that the identified proteins are involved in pathways of importance for glucose metabolism, inositol metabolism and calcium-dependent signaling thereby adding a novel facet to our understanding of cAMP-mediated potentiation of adipocyte differentiation.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Kamil Borkowski

LAB HEAD: Karsten Kristiansen

PROVIDER: PXD001063 | Pride | 2014-09-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1 FtTuCxswm.dat-pride.xml-merged.pride.mgf.gz	Mgf
	1 FtTuCxswm.dat-pride.xml-merged.pride.mztab.gz	Mztab
	1FtTuCxswm.dat-pride.xml-merged.xml.gz	Xml
	MGF.zip	Other
	Mascotsearchdata.zip	Other

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Publications

Proteomic analysis of cAMP-mediated signaling during differentiation of 3 T3-L1 preadipocytes.

Borkowski Kamil K Wrzesinski Krzysztow K Rogowska-Wrzesinska Adelina A Audouze Karine K Bakke Jesse J Petersen Rasmus Koefoed RK Haj Fawaz G FG Madsen Lise L Kristiansen Karsten K

Biochimica et biophysica acta 20140822 12

Initiation of adipocyte differentiation is promoted by the synergistic action of insulin/insulin-like growth factor, glucocorticoids, and agents activating cAMP-dependent signaling. The action of cAMP is mediated via PKA and Epac, where at least part of the PKA function relates to strong repression of Rho kinase activity, whereas Epac counteracts the reduction in insulin/insulin-like growth factor signaling associated with complete repression of Rho kinase activity. However, detailed knowledge o ...[more]

PMID: 25152230

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