Dataset Information

Interaction of cGMP-dependent protein kinase and HAP1

ABSTRACT: Here, we use cAMP-based chemical proteomics approach, employing competitive concentrations of free cyclic nucleotides to isolate PKA and PKG and their secondary interactors.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Lung, Permanent Cell Line Cell

SUBMITTER: Eleonora Corradini

LAB HEAD: Albert J.R. Heck

PROVIDER: PXD001434 | Pride | 2015-02-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	OR2_110709_EC_MIP_PKApulldown_pluscGMP1.RAW	Raw
	OR2_110709_EC_MIP_PKApulldown_pluscGMP2.RAW	Raw
	OR2_110709_EC_MIP_PKApulldown_pluscGMP3.RAW	Raw
	OR2_110709_EC_MIP_PKApulldown_pluscGMP4.RAW	Raw
	OR2_110709_EC_MIP_PKGpulldown_HEK293_control.msf	Msf

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Publications

Huntingtin-associated protein 1 (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP) specific for the cGMP-dependent protein kinase Iβ isoform.

Corradini Eleonora E Burgers Pepijn P PP Plank Michael M Heck Albert J R AJ Scholten Arjen A

The Journal of biological chemistry 20150204 12

Protein-protein interactions are important in providing compartmentalization and specificity in cellular signal transduction. Many studies have hallmarked the well designed compartmentalization of the cAMP-dependent protein kinase (PKA) through its anchoring proteins. Much less data are available on the compartmentalization of its closest homolog, cGMP-dependent protein kinase (PKG), via its own PKG anchoring proteins (GKAPs). For the enrichment, screening, and discovery of (novel) PKA anchoring ...[more]

PMID: 25653285

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