Dataset Information

Entamoeba histolytica splicing/mRNP proteome

ABSTRACT: First, the snRNP component U1A was HA-tagged and the intron of the RabX13 gene was MS2-tagged and amoeba transformats were established with such constructs. Next, amoeba transformants were UV cross-linked and their nucler extracts were immunoprecipitated (IP) with anti-HA agarose or MS2-spharose beads. Precipitates were subjected to tandem mass spectrometry (MS/MS) analyses. MS2-IP helped to discriminate the nuclear roles (chromatin-, co-transcriptional-, splicing-related), of the proteins probed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Entamoeba Histolytica

SUBMITTER: Robert Winkler

LAB HEAD: Dr. Jesus Valdes Flores

PROVIDER: PXD001080 | Pride | 2014-09-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	interact-mock1.pep.xml	Pepxml
	interact-mock1.prot.xml	Xml
	interact-mock2.pep.xml	Pepxml
	interact-mock2.prot.xml	Xml
	interact-u1abatch1.pep.xml	Pepxml

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Publications

Proteomic analysis of Entamoeba histolytica in vivo assembled pre-mRNA splicing complexes.

Valdés Jesús J Nozaki Tomoyoshi T Sato Emi E Chiba Yoko Y Nakada-Tsukui Kumiko K Villegas-Sepúlveda Nicolás N Winkler Robert R Azuara-Liceaga Elisa E Mendoza-Figueroa María Saraí MS Watanabe Natsuki N Santos Herbert J HJ Saito-Nakano Yumiko Y Galindo-Rosales José Manuel JM

Journal of proteomics 20140808

The genome of the human intestinal parasite Entamoeba histolytica contains nearly 3000 introns and bioinformatic predictions indicate that major and minor spliceosomes occur in Entamoeba. However, except for the U2-, U4-, U5- and U6 snRNAs, no other splicing factor has been cloned and characterized. Here, we HA-tagged cloned the snRNP component U1A and assessed its expression and nuclear localization. Because the snRNP-free U1A form interacts with polyadenylate-binding protein, HA-U1A immunoprec ...[more]

PMID: 25109466

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