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MicroRNA-Directed siRNA Biogenesis in Caenorhabditis elegans

ABSTRACT: C.elegans small RNAs from HA::ALG-1, HA::ALG-2 and HA::RDE-1 IP and rde-1 mutants Small RNAs were cloned from transgenic or mutant C. elegans adults. Sequencing was performed using 454 and Illumina platforms.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Eugene Berezikov

PROVIDER: E-GEOD-20649 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-directed siRNA biogenesis in Caenorhabditis elegans.

Corrêa Régis L RL Steiner Florian A FA Berezikov Eugene E Ketting René F RF

PLoS genetics 20100408 4

RNA interference (RNAi) is a post-transcriptional silencing process, triggered by double-stranded RNA (dsRNA), leading to the destabilization of homologous mRNAs. A distinction has been made between endogenous RNAi-related pathways and the exogenous RNAi pathway, the latter being essential for the experimental use of RNAi. Previous studies have shown that, in Caenorhabditis elegans, a complex containing the enzymes Dicer and the Argonaute RDE-1 process dsRNA. Dicer is responsible for cleaving ds ...[more]

PMID: 20386745

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