Dataset Information

The Cystatin D Interactome Associated to Systemic Mastocytosis

ABSTRACT: he aim of the project is to characterize qualitatively and quantitatively the interaction of cystatin D in other protein partner and the formation of a complex in whole saliva of patients affected by different forms of systemic mastocytosis (SM), with and without skin involvement. The different composition of the complex could be useful to distinguish patients from healthy subject and the different forms of systemic mastocytosis. Pools of whole saliva from SM (SM-C and SM+C) patients, and from sex/age matched healthy controls were submitted to immunoprecipitation assay using cystatin D antibody followed by SDS-PAGE in reducing conditions, tryptic digestion, and nano-HPLC-high-resolution-MS/MS analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Systemic Mastocytosis

SUBMITTER: Simone Serrao

LAB HEAD: Professor Tiziana Cabras

PROVIDER: PXD042567 | Pride | 2023-10-24

REPOSITORIES: Pride

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Publications

Salivary Cystatin D Interactome in Patients with Systemic Mastocytosis: An Exploratory Study.

Serrao Simone S Contini Cristina C Guadalupi Giulia G Olianas Alessandra A Lai Greca G Messana Irene I Castagnola Massimo M Costanzo Giulia G Firinu Davide D Del Giacco Stefano S Manconi Barbara B Cabras Tiziana T

International journal of molecular sciences 20230927 19

Mastocytosis, a rare blood disorder characterized by the proliferation of clonal abnormal mast cells, has a variegated clinical spectrum and diagnosis is often difficult and delayed. Recently we proposed the cathepsin inhibitor cystatin D-R<sub>26</sub> as a salivary candidate biomarker of systemic mastocytosis (SM). Its C<sub>26</sub> variant is able to form multiprotein complexes (mPCs) and since protein-protein interactions (PPIs) are crucial for studying disease pathogenesis, potential marke ...[more]

PMID: 37834061

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	Ctrls.mgf	Mgf
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	Ctrls_1.raw	Raw