Dataset Information

Proteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking

ABSTRACT: Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron oxide polystyrene nanoparticles and analyzed their protein composition by label free quantitative mass spectrometry. The proteomic snapshot of organelle marker proteins revealed that an atypical macropinocytic-like mechanism mediated the entry of nanoparticles. We show that the entry mechanism is controlled by actin reorganization, atypical macropinocytic signaling and ADP-ribosylation factor 1. Additionally, our proteomics data demonstrated a central role for multivesicular bodies and multilamellar lysosomes in trafficking and final nanoparticle storage. This was confirmed by confocal microscopy and cryo-TEM measurements. By quantitatively analyzing the protein composition of nanoparticle-containing vesicles, our study clearly defines the routes of nanoparticle entry, intracellular trafficking and the proteomic milieu of a nanoparticle-containing vesicle.

INSTRUMENT(S): Synapt G2-S HDMS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Hela Cell

SUBMITTER: Stefan Tenzer

LAB HEAD: Stefan Tenzer

PROVIDER: PXD001175 | Pride | 2014-09-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Hofmannetal_peptide_quantification_report.csv	Csv
	Hofmannetal_protein_quantification_report.xlsx	Xlsx
	S121126_01.raw.zip	Raw
	S121126_02.raw.zip	Raw
	S121126_03.raw.zip	Raw

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Publications

Mass spectrometry and imaging analysis of nanoparticle-containing vesicles provide a mechanistic insight into cellular trafficking.

Hofmann Daniel D Tenzer Stefan S Bannwarth Markus B MB Messerschmidt Claudia C Glaser Simone-Franziska SF Schild Hansjörg H Landfester Katharina K Mailänder Volker V

ACS nano 20140929 10

Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron oxide polystyrene nanoparticles and analyzed their protein composition by label-free quantitative mass spectrometry. The proteomic snapshot of organelle marker proteins revealed that an atypical macropi ...[more]

PMID: 25244389

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