Proteomics

Dataset Information

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Exoproteome of Aspergillus flavus corneal isolates and saprophytes


ABSTRACT: A. flavus exoproteome reference map as well as identification of the exoproteins from corneal isolates.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Aspergillus Flavus (strain Atcc 200026 / Fgsc A1120 / Nrrl 3357 / Jcm 12722 / Srrc 167)

SUBMITTER: Naveen Luke  

LAB HEAD: Dharmalingam Kuppamuthu

PROVIDER: PXD001296 | Pride | 2015-01-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AF_secretome_B11_15_1.msf Msf
AF_secretome_B11_15_1.raw Raw
AF_secretome_B11_15_2.msf Msf
AF_secretome_B11_15_2.raw Raw
AF_secretome_B16_20_1.msf Msf
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Publications

Exoproteome of Aspergillus flavus corneal isolates and saprophytes: identification of proteoforms of an oversecreted alkaline protease.

Selvam Ramu Muthu RM   Nithya Rathnavel R   Devi Palraj Narmatha PN   Shree R S Bhuvana RS   Nila Murugesan Valar MV   Demonte Naveen Luke NL   Thangavel Chitra C   Maheshwari Jayapal Jeya JJ   Lalitha Prajna P   Prajna Namperumalsamy Venkatesh NV   Dharmalingam Kuppamuthu K  

Journal of proteomics 20141210


Aspergillus flavus infects the human eye leading to keratitis. Extracellular proteins, the earliest proteins that come in contact with the host and virulence related exoproteins, were identified in the fungus isolated from infected cornea. Virulence of the corneal isolates was tested in the Galleria mellonella larvae model and those isolates showing higher virulence were taken for subsequent exoproteome analysis. High resolution two-dimensional electrophoresis and mass spectrometry were used to  ...[more]

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