Dataset Information

Trichoderma RUT C-30 secretome after growth on different cellulosic substrates

ABSTRACT: Enzyme production by T. reesei Rut C-30 was studied in submerged cultures on five different cellulose-rich substrates, namely, commercial cellulose Avicel® and industrial-like cellulosic pulp substrates which consist mainly of cellulose, but also contain residual hemicellulose and lignin. In order to evaluate the hydrolysis of the substrates by the fungal enzymes, the spatial polymer distributions were characterised by cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C-NMR) in combination with spectral fitting. Proteins in culture supernatants at early and late stages of enzyme production were labeled by Tandem Mass Tags (TMT) and protein profiles were analysed by liquid chromatography-tandem mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Trichoderma Reesei Rut C-30

TISSUE(S): Culture Supernatant, Fungal Cell

SUBMITTER: George Anasontzis

LAB HEAD: Lisbeth Olsson

PROVIDER: PXD001304 | Pride | 2014-09-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1654Set1TMT_JGI50mmuIT140822QE_130909_30.msf	Msf
	1654Set2TMT_JGI50mmuIT140822QE_130922_77.msf	Msf
	1654Set3TMT_JGI50mmuIT140822QE_130930_23.msf	Msf
	1654set1JGIrep1000mmu140205.mgf	Mgf
	1654set2JGIrep1000mmu140205.mgf	Mgf

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Publications

Morphology and enzyme production of Trichoderma reesei Rut C-30 are affected by the physical and structural characteristics of cellulosic substrates.

Peciulyte Ausra A Anasontzis George E GE Karlström Katarina K Larsson Per Tomas PT Olsson Lisbeth L

Fungal genetics and biology : FG & B 20140802

The industrial production of cellulolytic enzymes is dominated by the filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina). In order to develop optimal enzymatic cocktail, it is of importance to understand the natural regulation of the enzyme profile as response to the growth substrate. The influence of the complexity of cellulose on enzyme production by the microorganisms is not understood. In the present study we attempted to understand how different physical and structural pr ...[more]

PMID: 25093270

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