Dataset Information

Rapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer

ABSTRACT: The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide fractionation and single-shot phosphoproteomics are evaluated.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Christian Kelstrup

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD001305 | Pride | 2014-10-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	RawFilesforDilutionSeriesMethodComparison.zip	Other
	RawFilesforInstrumentandIsolationWidthComparison.zip	Other
	RawFilesforProteomeHpH-reversedPhase.zip	Other
	RawFilesforSingleShotPhosphoproteome.zip	Other
	Results.zip	Other

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Publications

Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer.

Kelstrup Christian D CD Jersie-Christensen Rosa R RR Batth Tanveer S TS Arrey Tabiwang N TN Kuehn Andreas A Kellmann Markus M Olsen Jesper V JV

Journal of proteome research 20141110 12

Shotgun proteomics is a powerful technology for global analysis of proteins and their post-translational modifications. Here, we investigate the faster sequencing speed of the latest Q Exactive HF mass spectrometer, which features an ultra-high-field Orbitrap mass analyzer. Proteome coverage is evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments (ProteomeXchange data set PXD001305). We find the ultra-high-field Orbitrap mass analyzer ...[more]

PMID: 25349961

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