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Temporal quantitative proteomics of Chikungunya virus infected cells

ABSTRACT: To better understand the complex CHIKV-host interplay we used stable isotope labeling with amino acids in cell culture (SILAC) and LC-MS/MS to define the temporal dynamics of the cellular response to infection. Using samples harvested at 8, 10 and 12 hours post infection, over 4700 proteins were identified and per time point 2750-3150 proteins could be quantified in both biological replicates.

INSTRUMENT(S): LTQ FT Ultra

ORGANISM(S): Cercopithecidae (old World Monkeys)

TISSUE(S): Cell Culture

DISEASE(S): Chikungunya

SUBMITTER: Emmely Treffers

LAB HEAD: Peter A van Veelen

PROVIDER: PXD001330 | Pride | 2015-03-16

REPOSITORIES: Pride

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			Action	DRS
	CHIKV_LS3.FASTA	Fasta
	human.first.search.fasta	Fasta
	ipi.HUMAN.v3.72.fasta	Fasta
	maxquant_output.zip	Other
	s2113009a.RAW	Raw

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Publications

Temporal SILAC-based quantitative proteomics identifies host factors involved in chikungunya virus replication.

Treffers Emmely E EE Tas Ali A Scholte Florine E M FE Van Myrthe N MN Heemskerk Matthias T MT de Ru Arnoud H AH Snijder Eric J EJ van Hemert Martijn J MJ van Veelen Peter A PA

Proteomics 20150422 13

Chikungunya virus (CHIKV) is an arthropod-borne reemerging human pathogen that generally causes a severe persisting arthritis. Since 2005, the virus has infected millions of people during outbreaks in Africa, Indian Ocean Islands, Asia, and South/Central America. Many steps of the replication and expression of CHIKV's 12-kb RNA genome are highly dependent on cellular factors, which thus constitute potential therapeutic targets. SILAC and LC-MS/MS were used to define the temporal dynamics of the ...[more]

PMID: 25764339

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