Proteomics

Dataset Information

0

Human Transcription Factors LC-MSMS


ABSTRACT: How transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated via protein-protein interactions remains elusive. To systematically investigate the regulations and functions of human transcription factors, we performed proteomics studies of soluble and chromatin-associated complexes of 56 TFs in HEK293T cells, using tandem-affinity-purification followed by mass spectrometry (TAP/MS). We performed 214 purifications and identified 2,156 high-confident protein-protein interactions.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Early Embryonic Cell

DISEASE(S): Disease Free

SUBMITTER: Xu Li  

LAB HEAD: Junjie Chen

PROVIDER: PXD001383 | Pride | 2015-04-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
21877.RAW Raw
21877.pride.mgf.gz Mgf
21877.pride.mztab.gz Mztab
21877.xml.gz Xml
21878.RAW Raw
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Publications

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.

Li Xu X   Wang Wenqi W   Wang Jiadong J   Malovannaya Anna A   Xi Yuanxin Y   Li Wei W   Guerra Rudy R   Hawke David H DH   Qin Jun J   Chen Junjie J  

Molecular systems biology 20150121 1


The current knowledge on how transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated by protein-protein interactions remains limited. Here, we performed proteomics analyses of soluble and chromatin-associated complexes of 56 TFs, including the targets of many signalling pathways involved in development and cancer, and 37 members of the Forkhead box (FOX) TF family. Using tandem affinity purification followed by mass spectrometry (TAP/MS), we  ...[more]

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