Dataset Information

Carbonylation proteome of rice embryo

ABSTRACT: Using affinity chromatography to enrich carbonylated proteins from rice embryos germinated for 0, 24, 48, 72 h. Sequential window acquisition of all therotical spectra (SWATH) technology was used to quantify carbonylated proteins. Mass spectra data was searched to discover carbonylated peptides and their corresponding proteins.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Embryo

SUBMITTER: Zhang Hui

LAB HEAD: Pingfang Yang

PROVIDER: PXD001429 | Pride | 2016-02-01

REPOSITORIES: Pride

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Publications

Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH.

Zhang Hui H He Dongli D Yu Jianlan J Li Ming M Damaris Rebecca Njeri RN Gupta Ravi R Kim Sun Tae ST Yang Pingfang P

Proteomics 20160301 6

Seed germination is an important aspect of the plant life cycle, during which, reactive oxygen species (ROS) accumulate. The accumulation of ROS results in an increase in protein oxidation of which carbonylation is the most canonical one. However, there is insufficient information concerning protein oxidation, especially carbonylation and its contribution to seed germination. In this study, biotin hydrazide labeled chromatography combined with sequential window acquisition of all theoretical fra ...[more]

PMID: 26801057

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