Dataset Information

Membrane proteome of Bacillus spores

ABSTRACT: We carried out in-depth proteomic characterization of membrane proteins of Bacillus dormant and germinated spores. In addition, we relatively quantified the protein changes after spore germination. This project is aimed to identify common and species-specific germination related membrane proteins.

INSTRUMENT(S): 4800 Plus MALDI TOF/TOF

ORGANISM(S): Bacillus Anthracis Str. Sterne Bacillus Subtilis Subsp. Subtilis Str. 168

TISSUE(S): Spore, Endospore

SUBMITTER: Yan Chen

LAB HEAD: David L. Popham

PROVIDER: PXD002136 | Pride | 2019-02-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 221

Publications

Membrane Proteomes and Ion Transporters in Bacillus anthracis and Bacillus subtilis Dormant and Germinating Spores.

Chen Yan Y Barat Bidisha B Ray W Keith WK Helm Richard F RF Melville Stephen B SB Popham David L DL

Journal of bacteriology 20190225 6

Bacterial endospores produced by Bacillus and Clostridium species can remain dormant and highly resistant to environmental insults for long periods, but they can also rapidly germinate in response to a nutrient-rich environment. Multiple proteins involved in sensing and responding to nutrient germinants, initiating solute and water transport, and accomplishing spore wall degradation are associated with the membrane surrounding the spore core. In order to more fully catalog proteins ...[more]

PMID: 30602489

Similar Datasets

Project description:Spermatophyte pollen tubes and root hairs have been used as single-cell-type model systems toward understanding the molecular processes underlying polar growth of plant cells. Horsetail (Equisetum arvense L.) is a perennial herb species in Equisetopsida, which creates separately growing spring and summer stems in its life cycle. The mature chlorophyllous spores produced from spring stems can germinate without dormancy. Here we report the cellular features and protein expression patterns in five stages of horsetail spore germination (mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Using 2-DE combined with mass spectrometry, 80 proteins were found to be differentially expressed upon spore germination. Among them, proteins involved in photosynthesis, protein turnover, and energy supply were over-represented. Thirteen proteins appeared as isoforms on the gels, indicating the potential importance of post-translational modification. In addition, the dynamic changes of ascorbate peroxidase, peroxiredoxin, and dehydroascorbate reductase implied that reactive oxygen species homeostasis is critical in regulating cell division and tip-growth. The time course of germination and diverse expression patterns of proteins in photosynthesis, energy supply, lipid and amino acid metabolism indicated that heterotrophic and autotrophic metabolism were necessary in light-dependent germination of the spores. Twenty-six proteins were involved in protein synthesis, folding, and degradation, indicating that protein turnover is vital to spore germination and rhizoid tip-growth. Furthermore, the altered abundance of small G protein Ran1, 14-3-3 protein, actin, and Caffeoyl-CoA O-methyltransferase revealed that signaling transduction, vesicle trafficking, cytoskeleton dynamics, and cell wall modulation were critical to cell division and polar growth. These findings provide up-to-date evidences for understanding fern spore asymmetric division and rhizoid polar growth.

			Action	DRS
	B.aD1A.XML	Xml
	B.aD1B.XML	Xml
	B.aD1C.XML	Xml
	B.aD1D.XML	Xml
	B.aD1E.XML	Xml

Dataset Information

Membrane proteome of Bacillus spores

Dataset's files

Publications

Membrane Proteomes and Ion Transporters in <i>Bacillus anthracis</i> and <i>Bacillus subtilis</i> Dormant and Germinating Spores.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets