Dataset Information

Npr2-mediated cGMP signalling modulates S-palmitoylation and controls growth cone morphology of DRG neurons

ABSTRACT: A cGMP signalling cascade composed of the ligand C-type natriuretic peptide (CNP), the natriuretic peptide receptor 2 (Npr2) and the cGMP-dependent kinase Iα (cGKI) is implicated by growth cone splitting in the bifurcation of afferents from dorsal root ganglia (DRG), cranial sensory ganglia (CSG) and mesencephalic trigeminal neurons (MTN). To get further mechanistic insights into the process of axon bifurcation we applied different cell culture approaches to decipher downstream activities of cGKI in somatosensory growth cones. We demonstrate that CNP induces an enlargement of DRG growth cones via cGKI which can be blocked or stimulated by pharmacological reagents that interfere with S-palmitoylation. cGKI colocalizes primarily in the central domain of the growth cone with the palmitoylome and vesicular structures including the endoplasmic reticulum, early endosomes, lysosomes and in the soma in the Golgi apparatus. Consistently, an acyl-biotin-exchange chemistry-based screen indicated that cGMP signalling regulates S-palmitoylation of a restricted pool of proteins in the DRG-derived cell line F11.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Gunnar Dittmar

LAB HEAD: Gunnar Dittmar

PROVIDER: PXD010326 | Pride | 2018-10-18

REPOSITORIES: Pride

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			Action	DRS
	Waldorf_20150303_AD_RN_MSC11133_cyt_ctrl.raw	Raw
	Waldorf_20150303_AD_RN_MSC11134_cyt_ctrl2.raw	Raw
	Waldorf_20150303_AD_RN_MSC11135_cyt_ctrl3.raw	Raw
	Waldorf_20150303_AD_RN_MSC11136_mem_ctrl.raw	Raw
	Waldorf_20150303_AD_RN_MSC11137_mem_ctrl2.raw	Raw

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Publications

S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.

Dumoulin Alexandre A Dagane Alina A Dittmar Gunnar G Rathjen Fritz G FG

Frontiers in molecular neuroscience 20180924

Genetic investigations have demonstrated that a specific form of axonal branching - the bifurcation of afferents from dorsal root ganglia (DRG), cranial sensory ganglia (CSG) and mesencephalic trigeminal neurons (MTN) - is regulated by a cGMP-dependent signaling pathway. This cascade is composed of the ligand C-type natriuretic peptide (CNP), the receptor guanylyl cyclase Npr2, and the cGMP-dependent protein kinase Iα (cGKIα). In the absence of any one of these components, axons no longer bifurc ...[more]

PMID: 30319353

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