Proteomics

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Chronic exposure to chewing tobacco induces growth in normal oral cells via overexpression of Stearoyl-CoA desaturase

ABSTRACT: The practice of using chewing tobacco is common in certain socio-economic sections of southern Asia particularly in the Indian subcontinent. The molecular mechanism of smokeless tobacco which leads to malignancy is unclear. Chewing tobacco demonstrates a carcinogenic effect through chronic and not acute exposures. Using a cell line model, we studied the chronic effects of chewing tobacco on the proteome in normal oral keratinocytes (OKF6/TERT1). We carried out iTRAQ-based quantitative proteomic analysis of the untreated and chewing tobacco treated oral keratinocytes. LC-MS/MS analysis of this cell line pair resulted in the identification of 3,638 proteins of which 408 were found to be differentially expressed.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Okf-6 Cell, Keratinized Cell Of The Oral Mucosa

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD001554 | Pride | 2016-12-14

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
OKF6_MultiConsensus.pep.xml Pepxml
OKF6_TT_4plex_iTRAQ_10-02.msf Msf
OKF6_TT_4plex_iTRAQ_10.raw Raw
OKF6_TT_4plex_iTRAQ_11-01.msf Msf
OKF6_TT_4plex_iTRAQ_11.raw Raw
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Publications

Chewing tobacco is a common practice in certain socio-economic sections of southern Asia, particularly in the Indian subcontinent and has been well associated with head and neck squamous cell carcinoma. The molecular mechanisms of chewing tobacco which leads to malignancy remains unclear. In large majority of studies, short-term exposure to tobacco has been evaluated. From a biological perspective, however, long-term (chronic) exposure to tobacco mimics the pathogenesis of oral cancer more close  ...[more]

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