Proteomics

Dataset Information

0

Molecular alterations elicited by chronic exposure to cigarette smoke and chewing tobacco in oral keratinocytes


ABSTRACT: Tobacco is mainly consumed in two different forms (smoking and chewing) that vary in their composition and methods of intake. Despite being the leading cause of oral cancer, the molecular mechanisms resulting in malignancy upon tobacco exposure are yet to be fully elucidated. We therefore sought to compare the molecular alterations in oral keratinocytes exposed to smoke and chewing tobacco. OKF6/TERT1 cells were exposed to cigarette smoke condensate or chewing tobacco for progressively increasing durations (2, 4, 6 and 8 months). We employed a TMT-based quantitative proteomics approach to investigate the adverse effects of chronic cigarette smoke or chewing tobacco exposure in oral keratinocytes. LC/MS3 analysis resulted in the quantification of 5,342 proteins and 2,821 proteins in cigarette smoke and chewing tobacco exposed cells, respectively. Upstream regulator analysis indicates the involvement of distinct regulators in CSC exposed cells compared to STE exposed cells. In addition, exome sequencing revealed discrete genetic alterations in cells exposed to each insult. Current analysis defines a clear distinction in the molecular dysregulation in oral cells in response to different tobacco-based insults. Some of the proteins dysregulated in cigarette smoke or chewing tobacco exposed cells may serve as potential early detection biomarkers which could aid in stratification of patients based on tobacco usage history.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oral Epithelium

DISEASE(S): Oral Cavity Cancer

SUBMITTER: Aditi Chatterjee  

LAB HEAD: Aditi Chatterjee

PROVIDER: PXD007127 | Pride | 2019-11-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OKF6_CSC_R1_07.raw Raw
OKF6_CSC_R1_08.raw Raw
OKF6_CSC_R1_09.raw Raw
OKF6_CSC_R1_1.raw Raw
OKF6_CSC_R1_10.raw Raw
Items per page:
1 - 5 of 76
altmetric image

Publications


Tobacco usage is a known risk factor associated with development of oral cancer. It is mainly consumed in two different forms (smoking and chewing) that vary in their composition and methods of intake. Despite being the leading cause of oral cancer, molecular alterations induced by tobacco are poorly understood. We therefore sought to investigate the adverse effects of cigarette smoke/chewing tobacco exposure in oral keratinocytes (OKF6/TERT1). OKF6/TERT1 cells acquired oncogenic phenotype after  ...[more]

Similar Datasets

2018-03-05 | PXD007637 | Pride
2016-12-14 | PXD001554 | Pride
2016-12-14 | PXD003108 | Pride
2023-05-10 | PXD041174 | Pride
2016-10-12 | GSE82137 | GEO
2005-02-23 | GSE2302 | GEO
2019-02-21 | PXD011106 | Pride
2009-09-15 | E-GEOD-12586 | biostudies-arrayexpress
2008-03-04 | GSE10700 | GEO
2022-03-02 | PXD007108 | Pride