Proteomics,Multiomics

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Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium

ABSTRACT: Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found, that small ORFs (smORFs; <100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. The data highlights the minimal genome (33%, 269410 bp of M. pneumoniae genome), with an unexpected hidden layer of smORFs with essential functions.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mycoplasma Pneumoniae M29

SUBMITTER: Javier Delgado  

LAB HEAD: Luis Serrano

PROVIDER: PXD001611 | Pride | 2015-03-03

REPOSITORIES: pride

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Dataset's files

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Action DRS
01_120423_MLLS_MPN_15min_a-01.msf Msf
02_120428_MLLS_MPN_15m_a2.msf Msf
03_120423_MLLS_MPN_15m_b1.msf Msf
04_120430_MLLS_MPN_15m_b2.msf Msf
05_120423_MLLS_MPN_30min_a-01.msf Msf
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Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual do  ...[more]

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