Dataset Information

Proteomic analysis of dormancy induction in grapevine

ABSTRACT: Grapes are a valuable fruit and an important economic crop in the world, where wine production is a major industry. In grapevine, the environmental regulation of bud dormancy varies among its diverse genotypes. Certain grapevine genotypes become dormant in response to decreasing photoperiod and others require low temperature or both environmental cues to induce dormancy. This study used a proteomic approach to gain an understanding of the underlying molecular events involved in bud dormancy commitment.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Vitis Labrusca X Vitis Riparia Vitis Riparia (frost Grape) (vitis Vulpina) Vitis Riparia X Vitis Rupestris

TISSUE(S): Plant Cell, Shoot

DISEASE(S): Disease Free

SUBMITTER: Iniga George

LAB HEAD: Paul A Haynes

PROVIDER: PXD001627 | Pride | 2018-06-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F2110Longday28daysReplicate1RAW.zip	Other
	F2110Longday28daysReplicate1mzxml.zip	Other
	F2110Longday28daysReplicate2RAW.zip	Other
	F2110Longday28daysReplicate2mzxml.zip	Other
	F2110Longday28daysReplicate3RAW.zip	Other

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Publications

Shotgun proteomic analysis of photoperiod regulated dormancy induction in grapevine.

George Iniga S IS Fennell Anne Y AY Haynes Paul A PA

Journal of proteomics 20180529

Certain grapevine genotypes become dormant in response to decreasing photoperiod and others require low temperature or both environmental cues to induce dormancy. This study used a proteomic approach to gain an understanding of the underlying molecular events involved in bud dormancy commitment. Two F<sub>2</sub> siblings (F2-110 and F2-040) with differences in photoperiod induced dormancy responsiveness were subjected to long day (LD, 15 h, paradormancy maintenance or dormancy inhibition) or sh ...[more]

PMID: 29857064

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Project description:Bud dormancy is a critical developmental process for perennial plant survival, and also an important physiological phase that affects the next seasonM-bM-^@M-^Ys growth of temperate fruit trees. Bud dormancy is regulated by multiple genetic factors, and affected by various environmental factors, tree age and vigor. To understand molecular mechanism of bud dormancy in Japanese apricot (Prunus mume Sieb. et Zucc.), we constructed a custom oligo DNA microarray covering the Japanese apricot dormant bud ESTs referring to peach (P. persica) genome sequence. Because endodormancy release is a chilling temperature-dependent physiological event, genes showing chilling-mediated differential expression patterns are candidates to control endodormancy release. Using the microarray constructed in this study, we monitored gene expression changes of dormant vegetative buds of Japanese apricot during prolonged artificial chilling exposure. In addition, we analyzed seasonal gene expression changes. M-bM-^@M-^XNankoM-bM-^@M-^Y vegetative buds collected in November, and those exposed to chilling for 40 or 60 days were used as microarray samples. Among the 58539 different unigene probes, 2345 and 1059 genes were identified as being more than two-fold up-regulated and down-regulated, respectively, following chilling exposure for 60 days (P value < 0.05). The down-regulated genes included P. mume DORMANCY-ASSOCIATED MADS-box genes, which supported the previous quantitative RT-PCR and EST analyses showing that these genes are repressed by prolonged chilling treatments. The genes encoding lipoxygenase were remarkably up-regulated by prolonged chilling. Cluster analysis suggested that the expression of the genes showing expression changes by artificial chilling exposure were coordinately regulated by seasonal changes. Our parametric analysis of gene set enrichment suggested that genes related to jasmonic acid (JA) and oxylipin biosynthesis and metabolic processes were significantly up-regulated by prolonged chilling, whereas genes related to circadian rhythm were significantly down-regulated. The results obtained from the microarray analyses were verified by quantitative RT-PCR analysis of selected genes. Taken together, this study raised the possibility that the microarray platform constructed in this study is applicable for deeper understanding of molecular network related to agronomically important bud phisiologies including dormancy release. In this study, we used chilling exposed bud samples (0, 40, 60 days starting at November) and seasonal monthly bud samples (June to March). For the samples in dataset 1 (three different time points during chilling treatment), three technical replicates (60K M-CM-^W 3 per sample) with three biological replicates were averaged, whereas three technical replicates were averaged for the samples in dataset 2 (10 different seasonal time points)