Dataset Information

Immune suppressive neutrophils are a separate subset developing parallel to normal neutrophils

ABSTRACT: Release of neutrophil subsets (hypersegmented, mature and banded) into the bloodstream was evoked by acute inflammation induced by iv. Injection of 2ng/kg lipopolysaccharide in healthy human volunteers pulse (6 hrs) labelled with deuteriated glucose. Three hours after injection, the three neutrophil subsets were sorted from blood. Incorporation of label into DNA was measured and proteome profiles were determined using high-end proteomics techniques (dimethyl labeling, SCX, nano-uplc, Q-Exactive).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil, Blood

SUBMITTER: Patrick Wijten

LAB HEAD: Albert Heck

PROVIDER: PXD001674 | Pride | 2017-05-29

REPOSITORIES: pride

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			Action	DRS
	OR6_20140217_PW_Neutrophil_subpop_rep1_fr7.raw	Raw
	OR6_20140217_PW_Neutrophil_subpop_rep1_fr8.raw	Raw
	OR6_20140217_PW_Neutrophil_subpop_rep1_fr9.raw	Raw
	OR6_20140218_PW_Neutrophil_subpop_rep1_fr10-12.raw	Raw
	OR6_20140218_PW_Neutrophil_subpop_rep1_fr13-15.raw	Raw

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Publications

Human CD62L<sup>dim</sup> neutrophils identified as a separate subset by proteome profiling and in vivo pulse-chase labeling.

Tak Tamar T Wijten Patrick P Heeres Marjolein M Pickkers Peter P Scholten Arjen A Heck Albert J R AJR Vrisekoop Nienke N Leenen Luke P LP Borghans José A M JAM Tesselaar Kiki K Koenderman Leo L

Blood 20170517 26

During acute inflammation, 3 neutrophil subsets are found in the blood: neutrophils with a conventional segmented nucleus, neutrophils with a banded nucleus, and T-cell-suppressing CD62L<sup>dim</sup> neutrophils with a high number of nuclear lobes. In this study, we compared the in vivo kinetics and proteomes of banded, mature, and hypersegmented neutrophils to determine whether these cell types represent truly different neutrophil subsets or reflect changes induced by lipopolysaccharide (LPS) ...[more]

PMID: 28515092

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