Proteomics

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High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics

ABSTRACT: In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and performed in 96-well format. We combine this technology with in situ hepatic stimulation, to generate time-resolved maps of insulin signaling in the mouse liver.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Hepatocyte, Liver, Kidney

SUBMITTER: Mario Oroshi  

LAB HEAD: Matthias Mann

PROVIDER: PXD001792 | Pride | 2015-08-19

REPOSITORIES: Pride

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1_QEp2_LC11_SeHu_LivPhos_EarlyInsulin_10s_Bio1.raw Raw
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High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.

Humphrey Sean J SJ   Azimifar S Babak SB   Mann Matthias M  

Nature biotechnology 20150817 9

Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes in diverse cells and tissues at a depth of >10,000 sites. We apply this technology to generate time-resolved maps of insulin signaling in the mouse liver. Our results reveal that ins  ...[more]

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