Proteomics

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Kefir peptide profiling - Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins

ABSTRACT: This study examines the proteolytic activity of the kefir grains (a combination of bacteria and yeast) on bovine milk proteins. SDS-PAGE analysis reveals substantial digestion of milk proteins by the kefir grains in comparison with control samples. Mass spectrometric analysis reveals that the kefir microorganisms released 609 new peptide fragments and significantly altered the abundance of around 1,500 peptides compared to the controls. These kefir-digested peptides derived from 55 milk proteins. We show that kefir contains 25 previously identified functional peptides with actions including antihypertensive, antimicrobial, opioid and anti-oxidative .

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Mammary Epithelial Cell, Mammary Gland Epithelial Cell

SUBMITTER: David Dallas  

LAB HEAD: David Dallas

PROVIDER: PXD001826 | Pride | 2016-05-09

REPOSITORIES: Pride

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Action DRS
GPM22200001207.xml-pride.pride.mgf.gz Mgf
GPM22200001207.xml-pride.pride.mztab.gz Mztab
GPM22200001207.xml-pride.xml.gz Xml
GPM22200001208.xml-pride.pride.mgf.gz Mgf
GPM22200001208.xml-pride.pride.mztab.gz Mztab
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Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins.

Dallas David C DC   Citerne Florine F   Tian Tian T   Silva Vitor L M VL   Kalanetra Karen M KM   Frese Steven A SA   Robinson Randall C RC   Mills David A DA   Barile Daniela D  

Food chemistry 20151026 Pt A

<h4>Scope</h4>The microorganisms that make up kefir grains are well known for lactose fermentation, but the extent to which they hydrolyze and consume milk proteins remains poorly understood. Peptidomics technologies were used to examine the proteolytic activity of kefir grains on bovine milk proteins.<h4>Methods and results</h4>Gel electrophoresis revealed substantial digestion of milk proteins by kefir grains, with mass spectrometric analysis showing the release of 609 protein fragments and al  ...[more]

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