Dataset Information

Soybean Suspension culture analysis

ABSTRACT: Proteomic analysis of soybean suspension cell extracellular and intracellular protein

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Jan Miernyk

LAB HEAD: Jan A. Miernyk

PROVIDER: PXD001895 | Pride | 2022-02-22

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ExtracellularTC-Day5.RAW	Raw
	ExtracellularTC-Day5.mzid.gz	Mzid
	ExtracellularTC-Day5.pride.mztab.gz	Mztab
	Intercellular JackTC.pride.mztab.gz	Mztab
	IntercellularJackTC.RAW	Raw

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Publications

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis.

Miernyk Ján A JA Jett Alissa A AA Johnston Mark L ML

Journal of proteomics 20150903

<h4>Unlabelled</h4>Excised hypocotyls from developing soybean (Glycine max (L.) merr. cv. Jack) were cultivated on agar-solidified medium until callus formed. The calli were then propagated in liquid medium until stable, relatively uniform, finely-divided suspension cultures were obtained. Cells were typically transferred to fresh medium at 7-day intervals. Cultures were harvested by filtration five days (early log phase) or eight days (late log phase) after transfer. In order to evaluate dynami ...[more]

PMID: 26344131

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