Proteomics

Dataset Information

0

Proteomic Analysis of a Soybean Suspension Culture


ABSTRACT: A developmental analysis of intercellular and extracellular proteins from soybean suspension cells through a culture cycle

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Cell Culture

SUBMITTER: Jan Miernyk  

LAB HEAD: Jan A. Miernyk

PROVIDER: PXD001172 | Pride | 2022-02-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2Dgelspot-1.d.zip Other
2Dgelspot-1.mzid.gz Mzid
2Dgelspot-1.mzid_2Dgelspot-1.MGF Mzid
2Dgelspot-1.mzid_2Dgelspot-1.pride.mgf.gz Mzid
2Dgelspot-1.pride.mztab.gz Mztab
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Publications

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis.

Miernyk Ján A JA   Jett Alissa A AA   Johnston Mark L ML  

Journal of proteomics 20150903


<h4>Unlabelled</h4>Excised hypocotyls from developing soybean (Glycine max (L.) merr. cv. Jack) were cultivated on agar-solidified medium until callus formed. The calli were then propagated in liquid medium until stable, relatively uniform, finely-divided suspension cultures were obtained. Cells were typically transferred to fresh medium at 7-day intervals. Cultures were harvested by filtration five days (early log phase) or eight days (late log phase) after transfer. In order to evaluate dynami  ...[more]

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