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Analysis of pre-and postsynaptic brain receptors, transporters and channels by mass spectrometry

ABSTRACT: Although receptors, transporters and channels (RTCs) are key elements in the synaptic membrane, work on analysis and quantification of RTCs by mass spectrometry is limited. Additionally, there is not a single mass spectrometrical study that assigns the localisation of RTCs to the pre- or post-synaptic membrane. Determination of localisation and levels of RTCs in pre- or post-synaptic membranes are essential as RTCs may show different functions in different locations.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Fernando J. Sialana

LAB HEAD: Gert Lubec

PROVIDER: PXD001948 | Pride | 2016-12-12

REPOSITORIES: pride

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			Action	DRS
	20150102-TMT6plex.msf	Msf
	M489-A01-O229-T104S1-P6183-1.raw	Raw
	M489-A02-O229-T104S1-P6183-1.raw	Raw
	M489-A03-O229-T104S1-P6183-1.raw	Raw
	M489-A04-O229-T104S1-P6183-1.raw	Raw

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Publications

Mass spectrometric analysis of synaptosomal membrane preparations for the determination of brain receptors, transporters and channels.

Sialana Fernando J FJ Gulyassy Peter P Májek Peter P Sjöstedt Evelina E Kis Viktor V Müller André C AC Rudashevskaya Elena L EL Mulder Jan J Bennett Keiryn L KL Lubec Gert G

Proteomics 20161101 22

The molecular composition of synaptic signal transduction machineries shapes synaptic neurotransmission. The repertoire of receptors, transporters and channels (RTCs) comprises major signaling events in the brain. RTCs are conventionally studied by candidate immunohistochemistry and biochemistry, which are low throughput with resolution greatly affected by available immunoreagents and membrane interference. Therefore, a comprehensive resource of synaptic brain RTCs is still lacking. In particula ...[more]

PMID: 27759936

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