Proteomics

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Proteome Comparison of Different-Aged Cochlea

ABSTRACT: Our work describes the application of mass spectrometry-based label-free quantitative proteomics for the identification of differentially expressed proteins between different-aged cochlea. We report proteins exclusively present as well as up- and downregulated in the cochlear sensory epithelium at significant developmental stages. Additionally, we report proteins exclusively present on P3 that were recently identified in the cochlea for the first time. We use bioinformatics to provide insight on biological functions and potential primary and secondary partners of the differentially expressed proteins. This study provides the first differentially expressed proteome in the mammalian cochlea at significant developmental stages; before hearing, during the onset of hearing, and when hearing is fully developed. We believe that these results will provide insights into the function of proteins that change during development and identify potential protein biomarkers related to hearing impairments.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Sensory Epithelial Cell, Cochlea

DISEASE(S): Disease Free

SUBMITTER: Lancia Darville  

LAB HEAD: Bernd Sokolowski

PROVIDER: PXD001973 | Pride | 2018-08-29

REPOSITORIES: Pride

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Publications

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Darville Lancia N F LNF   Sokolowski Bernd H A BHA  

Proteome science 20180807

<h4>Background</h4>The sensory epithelium of the inner ear converts the mechanical energy of sound to electro-chemical energy recognized by the central nervous system. This process is mediated by receptor cells known as hair cells that express proteins in a timely fashion with the onset of hearing.<h4>Methods</h4>The proteomes of 3, 14, and 30 day-old mice cochlear sensory epithelia were revealed, using label-free quantitative mass spectrometry (LTQ-Orbitrap). Statistical analysis using a one-wa  ...[more]

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