Dataset Information

Cell surface glycoproteome liver and lung

ABSTRACT: Purpose Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue. Experimental design We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture-specific approach combined with a robust bioinformatics filtering. Results Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte-specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver. Conclusions and clinical relevance We demonstrate the feasibility and the importance of conducting such studies directly in a clinically-relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Lung, Liver, Epithelial Cell, Cell Culture

SUBMITTER: Axel Ducret

LAB HEAD: Axel Ducret

PROVIDER: PXD001988 | Pride | 2015-07-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1ST_A549_COMBINED_5MM_BIOCYTIN.xls	Xls
	1ST_A549_COMBINED_NO_BIOCYTIN.xls	Xls
	1ST_A549_ELUAT_5MM_BIOCYTIN.RAW	Raw
	1ST_A549_ELUAT_5MM_BIOCYTIN.xls	Xls
	1ST_A549_ELUAT_NO_BIOCYTIN.RAW	Raw

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Publications

Identification of six cell surface proteins for specific liver targeting.

Ducret Axel A Kux van Geijtenbeek Sabine S Röder Daniel D Simon Sandrine S Chin Daniel D Berrera Marco M Gruenbaum Lore L Ji Changhua C Cutler Paul P

Proteomics. Clinical applications 20150801 7-8

<h4>Purpose</h4>Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue.<h4>Experim ...[more]

PMID: 26097162

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