Dataset Information

Compartmentalisation proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation

ABSTRACT: The emerging roles of endolysosomes (EL) in AF have yet to be discovered, especially how the EL contribute to the development of AF within a slow time frame. To identify the EL role in AF, we applied proteomics, transcriptomics, integrated analysis, electron tomography, western blotting and enzyme assays. To understand the cellular and EL driven dominant pathways, in AF, we applied an organelle protein isolation method, that was previously developed by us (Ayagama et al,.2021) on to an AF goat model. Our findings include upregulation of AMPK pathway, EL-specific proteins that were not identified in the TL, such as GAA, DYNLRB1, CLTB, SIRT3, CCT2, and muscle specific HSPB2. The structural anomalies such as autophago-vacuole formation, irregularly stacked mitochondria, and glycogen deposition provide an insight into of the contribution of EL contribution and the related pathways and molecular mechanisms.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Capra Hircus X Ovis Aries

TISSUE(S): Heart

SUBMITTER: Georgina Berridge

LAB HEAD: Rebecca AB Burton

PROVIDER: PXD041056 | Pride | 2024-06-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	FL1266_MSQ1984_202008_ThamaliA_10_EL_3032016.raw	Raw
	FL1266_MSQ1984_202008_ThamaliA_11_EL_41566.raw	Raw
	FL1266_MSQ1984_202008_ThamaliA_12_EL_38658.raw	Raw
	FL1266_MSQ1984_202008_ThamaliA_13_EL_37703.raw	Raw
	FL1266_MSQ1984_202008_ThamaliA_14_EL_33246.raw	Raw

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Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation.

Ayagama Thamali T Charles Philip D PD Bose Samuel J SJ Boland Barry B Priestman David A DA Aston Daniel D Berridge Georgina G Fischer Roman R Cribbs Adam P AP Song Qianqian Q Mirams Gary R GR Amponsah Kwabena K Heather Lisa L Galione Antony A Herring Neil N Kramer Holger H Capel Rebecca A RA Platt Frances M FM Schotten Ulrich U Verheule Sander S Burton Rebecca A B RAB

iScience 20240328 6

Endolysosomes (EL) are known for their role in regulating both intracellular trafficking and proteostasis. EL facilitate the elimination of damaged membranes, protein aggregates, membranous organelles and play an important role in calcium signaling. The specific role of EL in cardiac atrial fibrillation (AF) is not well understood. We isolated atrial EL organelles from AF goat biopsies and conducted a comprehensive integrated omics analysis to study the EL-specific proteins and pathways. We also ...[more]

PMID: 38827406

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