Proteomics

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Phosphoproteomic analysis of methanoarchaeon Methanohalophilus portucalensis FDF1T reveals diverse functions in methanogenesis and osmoadaptation

ABSTRACT: Methane is a well-known factor in green house effect and also could be an alternative energy, which produced via methanogenesis by methanogenic archaea. Methanohalophilus portucalensis FDF1T is a halophilic methylotrophic methanogen and has been used as a model to study osmoadaptation in fluctuated abiotic environmental conditions. Here we report a genome-wide phosphoproteomic analysis of M. portucalensis FDF1T to uncover the possible phosphorylation-mediated regulations in methanogenesis and osmoadaptation. According to the biological function analysis revealed 20.6% of total 149 phosphoproteins were involved in the regulation of the single carbon metabolism for methanogenesis and osmoadaptation. Together with genome sequencing and phosphoproteomic analysis, we provided the first evidence to indicate protein phosphorylation networks in regulating the pyrrolysine (Pyl) mediated translation of methylotrophic methyltransferase. Furthermore, the functional assays of glycine sarcosine N-methyltransferase revealed that the enzymatic activity was modulated by threonine phosphorylation. Collectively, our results not only provide new insight into phosphorylation-mediated regulations in single carbon energy metabolism but also clearly demonstrate that phosphorylation plays a critical role in osmolyte betaine synthesis through a phospho-switch mechanism.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Methanohalophilus

TISSUE(S): Cell Culture

SUBMITTER: Shu-Jung Lai  

LAB HEAD: Shu-Jung Lai

PROVIDER: PXD002024 | Pride | 2016-07-05

REPOSITORIES: Pride

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Publications

Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation.

Wu Wan-Ling WL   Lai Shu-Jung SJ   Yang Jhih-Tian JT   Chern Jeffy J   Liang Suh-Yuen SY   Chou Chi-Chi CC   Kuo Chih-Horng CH   Lai Mei-Chin MC   Wu Shih-Hsiung SH  

Scientific reports 20160630

Methanogens have gained much attention for their metabolic product, methane, which could be an energy substitute but also contributes to the greenhouse effect. One factor that controls methane emission, reversible protein phosphorylation, is a crucial signaling switch, and phosphoproteomics has become a powerful tool for large-scale surveying. Here, we conducted the first phosphorylation-mediated regulation study in halophilic Methanohalophilus portucalensis FDF1(T), a model strain for studying  ...[more]

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