Dataset Information

The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth

ABSTRACT: PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell wall biosynthesis. Here we demonstrate that over-expression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to -lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in biosynthesis of cell wall as revealed by proteomics studies. The growth inhibition caused by over-expression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell wall architecture.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycobacterium Smegmatis

TISSUE(S): Cell Culture

SUBMITTER: Andrew Bottrill

LAB HEAD: Galina V. Mukamolova

PROVIDER: PXD002120 | Pride | 2018-03-13

REPOSITORIES: Pride

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			Action	DRS
	150417_001_UL01836-OT-01.mzid.gz	Mzid
	150417_001_UL01836-OT-01.mzid_150417_001_UL01836-OT-01.MGF	Mzid
	150417_001_UL01836-OT-01.mzid_150417_001_UL01836-OT-01.pride.mgf.gz	Mzid
	150417_001_UL01836-OT-01.pride.mztab.gz	Mztab
	150417_001_UL01836-OT-01.raw	Raw

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The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth.

Turapov Obolbek O Loraine Jessica J Jenkins Christopher H CH Barthe Philippe P McFeely Daniel D Forti Francesca F Ghisotti Daniela D Hesek Dusan D Lee Mijoon M Bottrill Andrew R AR Vollmer Waldemar W Mobashery Shahriar S Cohen-Gonsaud Martin M Mukamolova Galina V GV

Open biology 20150701 7

PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell-wall biosynthesis. Here we demonstrate that overexpression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to β-lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in cell-wall biosynthesis as revealed by proteomics studies. The growth inhibition caused by ...[more]

PMID: 26136255

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