Proteomic characterization of the role of Snail1 in the differentiation of mesenchymal stem cell

ABSTRACT: Snail effects on adipogenesis was proposed to be mediated, among others, by a lack of response to TGFβ (Batlle et al., 2012) or by an apparent inhibition of PPARγ and C/EBPα expression (Lee et al., 2013). Similar results were obtained in preventing the differentiation of bone marrow-derived murine mesenchymal stem cells (mMSC) to osteoblasts or adipocytes (Batlle et al, 2012). Still, the molecular mechanisms underlying the effect of Snail on MSCs differentiation and the blocking of adipogenesis were far from being established. We carried out an in-depth quantitative proteomic analysis of Snail-transfected cells to identify the cellular and molecular mechanisms controlled by Snail. We used isobaric labeling with tandem mass tags (TMT) (Dayon and Sanchez, 2012) for mMSCs analysis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, Stem Cell, Cell Culture, Fibroblast, Bone Marrow

DISEASE(S): Colon Cancer

SUBMITTER: Alberto Pelaez García  

LAB HEAD: Functional Proteomics

PROVIDER: PXD002157 | Pride | 2015-09-08

REPOSITORIES: Pride