Proteomics

Dataset Information

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An effective plasma membrane proteomics approach for small tissue samples


ABSTRACT: Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of ? 1600 proteins of which > 60% are known to be associated to the plasma membrane according to GO annotations, including (G-protein coupled) receptors, ion channels and transporters, and this from mm3-scale tissue.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Dirk Valkenborg  

LAB HEAD: Dirk Valkenborg

PROVIDER: PXD002227 | Pride | 2022-02-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
fr1_ad98.msf Msf
fr1_ad98.raw Raw
fr1_ad98.xlsx Xlsx
fr1_ad99.msf Msf
fr1_ad99.raw Raw
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Publications

An effective plasma membrane proteomics approach for small tissue samples.

Smolders Katrien K   Lombaert Nathalie N   Valkenborg Dirk D   Baggerman Geert G   Arckens Lutgarde L  

Scientific reports 20150605


Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale  ...[more]

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