Proteomics

Dataset Information

0

Active Site Specificity Profiling of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by MMPs 1, 2, 3, 7, 8, 9, 12, 13, and 14.

ABSTRACT: Background: The active site of matrix metalloproteinases (MMPs) is highly conserved, complicating the rational design of specific substrates and inhibitors for individual family members. Results: Active site specificity profiles of 9 MMPs were determined using high throughput Proteomic Identification of protease Cleavage Sites (PICS). Conclusion: Subtle specificity divergences distinguish individual MMP family members. Significance: Understanding individual MMP cleavage site specificities will bolster the design of MMP specific activity assays and targeted inhibitory drugs.

INSTRUMENT(S): QSTAR XL, QSTAR Pulsar

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ulrich ECKHARD  

LAB HEAD: Prof. Christopher Mark Overall

PROVIDER: PXD002265 | Pride | 2015-09-29

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
MegaP_hMMP12_GluC_CB_QStar-XL_20080812.mzXML Mzxml
MegaP_hMMP12_GluC_CB_QStar-XL_20080812.tandem Other
MegaP_hMMP12_GluC_CB_QStar-XL_20080812.tandem.pep.xml Pepxml
MegaP_hMMP12_GluC_CB_QStar-XL_20080812.wiff Wiff
MegaP_hMMP12_Tryps_CB_QStar-XL_20080812.mzXML Mzxml
Items per page:
1 - 5 of 84
altmetric image

Publications

Active site specificity profiling of the matrix metalloproteinase family: Proteomic identification of 4300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses.

Eckhard Ulrich U   Huesgen Pitter F PF   Schilling Oliver O   Bellac Caroline L CL   Butler Georgina S GS   Cox Jennifer H JH   Dufour Antoine A   Goebeler Verena V   Kappelhoff Reinhild R   Keller Ulrich Auf dem UAD   Klein Theo T   Lange Philipp F PF   Marino Giada G   Morrison Charlotte J CJ   Prudova Anna A   Rodriguez David D   Starr Amanda E AE   Wang Yili Y   Overall Christopher M CM  

Matrix biology : journal of the International Society for Matrix Biology 20150925

Secreted and membrane tethered matrix metalloproteinases (MMPs) are key homeostatic proteases regulating the extracellular signaling and structural matrix environment of cells and tissues. For drug targeting of proteases, selectivity for individual molecules is highly desired and can be met by high yield active site specificity profiling. Using the high throughput Proteomic Identification of protease Cleavage Sites (PICS) method to simultaneously profile both the prime and non-prime sides of the  ...[more]

Similar Datasets

Proteomics Computational design of Matrix Metalloprotenaise-9 (MMP-9) resistant to auto-cleavage
2023-10-24 | PXD041767 | Pride
Proteomics Metalloprotease-mediated cleavage of CD95 Ligand: QTOF Xevo XS data
2023-02-20 | PXD039211 | Pride
Transcriptomics Proteolytic inactivation of the autoinhibitory prodomain is essential for the pro-tumorigenic function of membrane type-1 matrix metalloproteinase (MT1-MMP)
2011-06-01 | E-GEOD-28655 | biostudies-arrayexpress
Transcriptomics RNA-seq of Leishmania mexicana promastigotes, axenic amastigotes and intracellular amastigotes
2015-09-11 | E-MTAB-3312 | biostudies-arrayexpress
Transcriptomics microRNA profiling of HepG2 cells: control vs treatment with cacao, grape seed proanthocyanidin extract or epigallocatechin gallate
2014-07-01 | E-GEOD-30575 | biostudies-arrayexpress
Transcriptomics Genome-wide analysis of MCF-7 cells expressing or not MMP-14 (MT1-MMP) growing in monolayer cell culture conditions
2015-12-11 | E-GEOD-64320 | biostudies-arrayexpress
Proteomics ATOMS determination of MMP cleavage site locations in YRS
2019-05-29 | PXD013366 | Pride
Proteomics ATOMS analyses of MMP cleavage sites in WRS
2019-05-29 | PXD013367 | Pride
Proteomics Secretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function
2023-10-24 | PXD043521 | Pride
Transcriptomics Genome-wide analysis of control and MMP-14 (MT1-MMP) expressing MCF-7 cells growing in three-dimensional (3D) type I collagen gels versus monolayer cell culture conditions
2014-12-15 | E-GEOD-49595 | biostudies-arrayexpress