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Urinary polypeptide ETD/CID analysis, part 1

ABSTRACT: Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, to demonstrate that peptides are readily observed. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

DISEASE(S): Disease Free

SUBMITTER: Sarah Hart

LAB HEAD: Sarah Hart

PROVIDER: PXD002312 | Pride | 2016-02-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	T9082106.RAW	Raw
	T9082106_ITMSms2cid.txt (F001733).mzid_T9082106_ITMSms2cid.txt_(F001733).pride.mgf.gz	Mzid
	T9082106_ITMSms2cid.txt (F001733).pride.mztab.gz	Mztab
	T9082106_ITMSms2cid.txtF001733.mzid.gz	Mzid
	T9082106_ITMSms2cid.txtF001733.mzid_T9082106_ITMSms2cid.txt_F001733.MGF	Mzid

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Urinary polypeptide ETD/CID analysis, part 3

Project description:Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and polypeptidic protein degradation products, which have been filtered from blood plasma, thus urine has potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, and demonstrate ready observation of large polypeptide. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

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Urinary polypeptide ETD/CID analysis, part 1

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Project description:Urine as a biofluid is commonly used in clinical diagnostics, including those performed during pregnancy. Urine is a rich source of polypeptides and protein degradation products, which have been filtered from blood plasma, thus urine has strong potential as a source for novel clinical diagnostics in disease. In this study, we examine the urinary peptidome from normal healthy women during pregnancy, to demonstrate that peptides are readily observed. We utilise the dissociation method, electron transfer dissociation (ETD) to increase the identification rate of the peptides present within these samples, as the polypeptide species observed in these samples are large and highly charged. An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via the use of ETD.

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