Proteomics

Dataset Information

0

Quantitative proteomics of host-parasite interactions in Giardia duodenalis


ABSTRACT: Giardia duodenalis is the protozoan agent responsible for the majority of parasitic gastroenteritis in humans worldwide. Disease pathology includes malabsorption and maldigestion, cell apoptosis and small intestinal barrier dysfunction, which occurs in absence of known toxins, cell invasion and overt inflammation. To understand pathogenesis, host-parasite in vitro interaction models provide global insights into disease induction and virulence. Hence, we performed the first proteomic analysis of G. duodenalis trophozoites interacted with intestinal epithelial cells (IECs, HT-29) for 6 hours, and compared it to trophozoites exposed to cell-free fractions of host-soluble signals. This has allowed us to demonstrate that distinct and independent protein cascades are induced by host attachment compared to host soluble signals. We utilised a tandem mass tag (TMT) approach and evaluated it as quantitative proteomics for the first time in Giardia.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Giardia Intestinalis Assemblage A

TISSUE(S): Trophozoite

SUBMITTER: Samantha Emery  

LAB HEAD: Professor Paul Haynes

PROVIDER: PXD002398 | Pride | 2016-02-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
TMTFraction1.raw Raw
TMTFraction10.raw Raw
TMTFraction11.raw Raw
TMTFraction12.raw Raw
TMTFraction2.raw Raw
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Publications

Induction of virulence factors in Giardia duodenalis independent of host attachment.

Emery Samantha J SJ   Mirzaei Mehdi M   Vuong Daniel D   Pascovici Dana D   Chick Joel M JM   Lacey Ernest E   Haynes Paul A PA  

Scientific reports 20160212


Giardia duodenalis is responsible for the majority of parasitic gastroenteritis in humans worldwide. Host-parasite interaction models in vitro provide insights into disease and virulence and help us to understand pathogenesis. Using HT-29 intestinal epithelial cells (IEC) as a model we have demonstrated that initial sensitisation by host secretions reduces proclivity for trophozoite attachment, while inducing virulence factors. Host soluble factors triggered up-regulation of membrane and secrete  ...[more]

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