Multi-omics toxicity profiling of engineered nanomaterials
Ontology highlight
ABSTRACT: This study aims to implement methods for toxicological profiling of engineered nanomaterials using toxicogenomics, proteomics, and metabolomics along with computational analyses. For all three omics layers the human cell lines A549 (lung epithelial cells) and THP1 (monocytes) were separately exposed to the nanomaterials TiO2 NM104, MWNCT NM401, and Ag NM300k. Proteomics and metabolomics samples have been performed as biological triplicates and were taken after 0, 6, 12, 24, and 48 hours. To assess ecotoxic effects C. elegans worms were grown in soil treated with NM300k. Ecotox samples were taken only at 0 and 24 hours. Integrating all three omics layers will enable the identification of (novel) ENM specific modes of action (MoA).
INSTRUMENT(S): LTQ Orbitrap XL
ORGANISM(S): Homo Sapiens (human) Caenorhabditis Elegans
TISSUE(S): Monocyte, Epithelial Cell, Cell Culture
SUBMITTER: Marc Rurik
LAB HEAD: Christian Huber
PROVIDER: PXD002401 | Pride | 2016-07-25
REPOSITORIES: Pride
ACCESS DATA