Proteomics

Dataset Information

0

Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona

ABSTRACT: The increasing number of biological applications for black phosphorus (BP) nanomaterials has precipitated considerable concern about their interactions with physiological systems. Here, we demonstrate the adsorption of plasma protein onto BP nanomaterials and the subsequent immune perturbation effect on macrophages. Using liquid chromatography tandem mass spectrometry, we discovered that BP nanosheets (BPNSs) were able to bind 23.3 percent of immune proteins from plasma, while BP quantum dots (BPQDs) bound 41.8 percent of immune proteins. In particular, the protein corona dramatically reshaped BP nanomaterial-corona complexes, influenced cellular uptake, activated the NF-κB pathway and even increased cytokine secretion by 2-5-fold. BP nanomaterials induced immunotoxicity and immune perturbation in macrophages in the presence of a plasma corona. These findings offer important insights into the development of safe and effective BP nanomaterial-based therapies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Jianbin Mo  

LAB HEAD: Jing Zhao

PROVIDER: PXD009645 | Pride | 2018-05-18

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
P20170600181_Plasma-BPNS.dat Other
P20170600181_Plasma-BPNS.mgf Mgf
P20170600181_Plasma-BPNS.raw Raw
P20170600181_Plasma-BPQD.dat Other
P20170600181_Plasma-BPQD.mgf Mgf
Items per page:
1 - 5 of 9
altmetric image

Publications

Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona.

Mo Jianbin J   Xie Qingyun Q   Wei Wei W   Zhao Jing J  

Nature communications 20180626 1

The increasing number of biological applications for black phosphorus (BP) nanomaterials has precipitated considerable concern about their interactions with physiological systems. Here we demonstrate the adsorption of plasma protein onto BP nanomaterials and the subsequent immune perturbation effect on macrophages. Using liquid chromatography tandem mass spectrometry, 75.8% of the proteins bound to BP quantum dots were immune relevant proteins, while that percentage for BP nanosheet-corona compl  ...[more]

Similar Datasets

Proteomics Black Phosphorus-Corona LC-MS/MS
2018-03-07 | PXD009126 |
Proteomics New method to investigate protein orientation in corona of nanomaterials
2021-09-09 | PXD019524 | Pride
Transcriptomics Human granulosa
2014-07-14 | E-MTAB-2264 | biostudies-arrayexpress
Proteomics Plasma protein corona on superparamagnetic iron oxide nanoparticles (SPIONs)
2015-10-12 | PXD000766 | Pride
Proteomics Blood circulation of soft nanomaterials is governed by dynamic remodeling of protein opsonins at nanobiointerface
2020-05-03 | MSV000085364 | MassIVE
Proteomics Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules
2024-10-15 | PXD053359 | Pride
Proteomics Plasma Protein Corona on Polystyrene Nanoparticles Facilitates their Transfer across the Human Placenta
2020-08-19 | PXD018160 | Pride
Proteomics Mass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona
2024-10-15 | PXD050779 | Pride
Proteomics SPIO corona - Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles
2019-01-14 | PXD011781 | Pride
Transcriptomics Widespread Changes in Transcriptome Profile of Human Mesenchymal Stem Cells Induced by Two-Dimensional (2D) Nanosilicates
2018-03-31 | GSE108638 | GEO