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Serum glycoprotein biomarker discovery and qualification pipeline reveals novel diagnostic biomarkers for esophageal adenocarcinoma

ABSTRACT: The aim of this project is to identify circulatory diagnostic glycoprotein biomarkers for Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC). Serum samples from healthy, BE and EAC patients were screened using LeMBA-LC-MS/MS-GlycoSelector pipeline as described in the manuscript.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Barrett's Esophagus,Barrett's Adenocarcinoma

SUBMITTER: Alok Shah

LAB HEAD: Dr. Michelle M. Hill

PROVIDER: PXD002442 | Pride | 2015-09-25

REPOSITORIES: Pride

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			Action	DRS
	20120727_AShah_AAL-2.d.tar.bz2	Other
	20120727_AShah_BPL-2.d.tar.bz2	Other
	20120727_AShah_ConA-2.d.tar.bz2	Other
	20120727_AShah_Ctrl-2.d.tar.bz2	Other
	20120727_AShah_DSA-2.d.tar.bz2	Other

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Publications

Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma.

Shah Alok K AK Cao Kim-Anh Lê KA Choi Eunju E Chen David D Gautier Benoît B Nancarrow Derek D Whiteman David C DC Saunders Nicholas A NA Barbour Andrew P AP Joshi Virendra V Hill Michelle M MM

Molecular & cellular proteomics : MCP 20150924 11

We report an integrated pipeline for efficient serum glycoprotein biomarker candidate discovery and qualification that may be used to facilitate cancer diagnosis and management. The discovery phase used semi-automated lectin magnetic bead array (LeMBA)-coupled tandem mass spectrometry with a dedicated data-housing and analysis pipeline; GlycoSelector (http://glycoselector.di.uq.edu.au). The qualification phase used lectin magnetic bead array-multiple reaction monitoring-mass spectrometry incorpo ...[more]

PMID: 26404905

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