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Unknown,Transcriptomics,Genomics,Proteomics

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SOX15 governs a transcriptional program in human esophageal stratified epithelium and in a subset of esophageal adenocarcinomas

ABSTRACT: We identified spatially restricted transcription factors and found SOX15 expression confined to stratified esophageal epithelium, with attenuation in Barrett's esophagus. SOX15 binds esophagus-specific loci and its loss in human esophageal cells affected esophagus-specific transcripts [RNA-Seq] Total RNA isolated from CPA control cells and CPA cells following SOX15 depletion, samples were prepared for sequencing using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer's instructions. 75 base pair single-end reads were sequenced on an Illumina NextSeq 500 instrument. The data include 2 independent biological replicates per genotype. [ChIP-Seq] Examine SOX15-chromatin binding in CPA cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Ramesh Shivdasani

PROVIDER: E-GEOD-62909 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SOX15 governs transcription in human stratified epithelia and a subset of esophageal adenocarcinomas.

Sulahian Rita R Chen Justina J Arany Zoltan Z Jadhav Unmesh U Peng Shouyong S Rustgi Anil K AK Bass Adam J AJ Srivastava Amitabh A Hornick Jason L JL Shivdasani Ramesh A RA

Cellular and molecular gastroenterology and hepatology 20151101 6

<h4>Background & aims</h4>Intestinal metaplasia (Barrett's esophagus, BE) is the principal risk factor for esophageal adenocarcinoma (EAC). Study of the basis for BE has centered on intestinal factors, but loss of esophageal identity likely also reflects absence of key squamous-cell factors. As few determinants of stratified epithelial cell-specific gene expression are characterized, it is important to identify the necessary transcription factors.<h4>Methods</h4>We tested regional expression of ...[more]

PMID: 26516633

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