Proteomics

Dataset Information

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Phosphoproteomic Network Analysis Reveals Molecular Mechanisms of Myometrial Stretch Response


ABSTRACT: Mechanical stress is a potent regulator of cell growth, contractility and gene regulation. Abnormal uterine distension during pregnancy increases the risk of preterm birth and likely activates crosstalk between multiple signaling networks with protein phosphorylation playing a critical role. Telomerized human uterine smooth muscle cells were exposed to 18% biaxial stretch for 5 min and the phosphoproteome was probed by mass spectrometry. We observed specific phospho-activation of mitogen activated protein kinase at threonine 183 and tyrosine 185, myosin regulatory light chain 9 at threonine 19, and heat shock protein 27 at serine 82. Our analysis revealed protein phosphorylation changes in signaling pathways related to actin cytoskeleton remodeling, activation of the focal adhesion kinase pathway, smooth muscle contraction and mechanistic target of rapamycin activation. These data point to potential mechanistic links between stretch-induced phosphorylation and development of the contractile phenotype in myometrial cells.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myometrial Cell, Myometrium

SUBMITTER: Craig Ulrich  

LAB HEAD: Heather Burkin

PROVIDER: PXD002538 | Pride | 2019-04-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
01_TMT_PP.mzid.gz Mzid
01_TMT_PP.mzid_01_TMT_PP.MGF Mzid
01_TMT_PP.mzid_01_TMT_PP.pride.mgf.gz Mzid
01_TMT_PP.pride.mztab.gz Mztab
01_TMT_PP.raw Raw
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Publications

Mechanical strain induced phospho-proteomic signaling in uterine smooth muscle cells.

Copley Salem Christian C   Ulrich Craig C   Quilici David D   Schlauch Karen K   Buxton Iain L O ILO   Burkin Heather H  

Journal of biomechanics 20180330


Mechanical strain associated with the expanding uterus correlates with increased preterm birth rates. Mechanical signals result in a cascading network of protein phosphorylation events. These signals direct cellular activities and may lead to changes in contractile phenotype and calcium signaling. In this study, the complete phospho-proteome of uterine smooth muscle cells subjected to mechanical strain for 5 min was compared to un-strained controls. Statistically significant, differential phosph  ...[more]

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