Dataset Information

Western black widow major ampullate, minor ampullate, and tubuliform silk gland LC-MSMS, Part 2

ABSTRACT: Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq libraries identified 647 predicted genes that were differentially expressed in silk glands of the Western black widow, Latrodectus hesperus. Only ~5% of these transcripts encode spidroins and the remaining predicted genes presumably encode other proteins associated with silk production. Here, we used proteomic analysis of multiple silk glands and dragline silk fiber to investigate the translation of the differentially expressed genes. We find 48 proteins encoded by the differentially expressed transcripts in L. hesperus major ampullate, minor ampullate, and tubuliform silk glands, and detect 16 SST encoded proteins in major ampullate silk fibers. The observed proteins include known silk-related proteins, but most are uncharacterized, with no annotation. These unannotated proteins likely include novel silk associated proteins. Major ampullate and minor ampullate glands have the highest overlap of identified proteins, consistent with their shared, distinctive ampullate shape and the overlapping functions of major ampullate and minor ampullate silks. Our study substantiates and prioritizes predictions from differential expression analysis of spider silk gland transcriptomes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Latrodectus Hesperus (western Black Widow Spider)

TISSUE(S): Silk Gland

SUBMITTER: Ro Crystal Chaw

LAB HEAD: Cheryl Y Hayashi

PROVIDER: PXD002595 | Pride | 2015-08-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GeorgeTsaprailis-RCC_213215_LH3MAB.raw	Raw
	GeorgeTsaprailis-RCC_213215_LHMA12.raw	Raw
	GeorgeTsaprailis-RCC_213215_LH_MI.raw	Raw
	GeorgeTsaprailis-RCC_214032_LH4_TUB.raw	Raw
	GeorgeTsaprailis-RCC_214032_LH5_TUB.raw	Raw

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Publications

Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers.

Chaw Ro Crystal RC Correa-Garhwal Sandra M SM Clarke Thomas H TH Ayoub Nadia A NA Hayashi Cheryl Y CY

Journal of proteome research 20150901 10

Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous analyses of tissue-specific RNA-seq libraries identified 647 predicted genes that were differentially expressed in silk glands of the Western black widow, Latrodectus hesperus. Only ∼5% of these silk-gland specific transcripts (SSTs) enco ...[more]

PMID: 26302244

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