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LC-MS/MS data from spider major ampullate silk fibers and glands

ABSTRACT: This project contains proteomic (LC-MS/MS) data from 27 samples. The samples are as follows: (1) Major ampullate glands dissolved in 8M Urea (3 replicates); (2) Major ampullate silk fibers dissolved in 8M Urea (3 replicates); (3) Major ampullate silk fibers dissolved in Hexafluoroisopropanol (3 replicates); (4) Major ampullate silk fibers dissolved in 9M Lithium Bromide (3 replicates); (5) Major ampullate silk fibers dissolved in 2M Urea (3 replicates); (6) Major ampullate silk fibers dissolved in 4M Urea (3 replicates); (7) Major ampullate silk fibers dissolved in 8M Urea (3 replicates); (8) Major ampullate silk fibers first dissolved in Formic acid and then in 2M Urea (2 replicates); (9) Major ampullate silk fibers first dissolved in Formic acid and then in 4M Urea (2 replicates); (10) Major ampullate silk fibers first dissolved in Formic acid and then in 8M Urea (2 replicates);

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Larinioides Sclopetarius

SUBMITTER: Anna Rising

LAB HEAD: Anna Rising

PROVIDER: PXD052116 | Pride | 2024-07-16

REPOSITORIES: Pride

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Project description:Spider silk proteins are synthesized in the silk-producing glands, where the spidroins are produced, stored and processed into a solid fiber from a crystalline liquid solution. Despite great interest in the spider silk properties, that make this material suitable for biomedical and biotechnological applications, the mechanism of formation and spinning of the silk fibers has not been fully elucidated; and no combination of proteomic and transcriptomic study has been carried out so far in the spider silk-producing glands. Nephila clavipes is an attractive orb-web spider to investigate the spinning process of silk production, given the properties of strength, elasticity and biocompatibility of their silk fibers. Thus, considering that the combination of proteomic and transcriptomic analysis may reveal an extensive repertoire of novel proteins involved in the silk spinning process, and in order to facilitate and enable proteomics in this non-model organism, the current study aims to construct a high quality reference mRNA-derived protein database that could be used to identify tissue specific expression patterns in spider silk glands. Next-generation sequencing has offered a powerful and cost-efficient technique for the generation of transcriptomic datasets in non-model species using diverse platforms such as the Illumina HiSeq, Roche 454, Pacific Biosystems, and Applied Biosystems SOLiD; In the current study, the Illumina HiSeq 2000 platform will be used to generate a N. clavipes spider silk glands transcriptome-based protein database. The transcriptome data generated in this study will provide a comprehensive and valuable genomic resource for future research of the group of spider silk-producing glands, in order to improve our understanding of the overall mechanism of action involved in production, secretion, storage, transport, protection and conformational changes of spidroins during the spinning process, and prey capture; and the results may be relevant for scientists in material Science, biology, biochemistry, and environmental scientists.

			Action	DRS
	MA1_2Murea.raw	Raw
	MA1_4Murea.raw	Raw
	MA1_8Murea.raw	Raw
	MA2_2Murea.raw	Raw
	MA2_4Murea.raw	Raw

Dataset Information

LC-MS/MS data from spider major ampullate silk fibers and glands

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