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Proteomic and bioinformatic analysis of the nuclear intrinsically disordered proteome

ABSTRACT: Intrinsically disordered proteins (IDPs) are biologically active molecules which are involved in many cellular functions although they do not possess a defined three-dimensional structure. They are mostly signalling and regulatory proteins. This study is the first large-scale proteomic analysis of the nuclear IDPs. We experimentally showed that IDPs are overrepresented in the nucleus in comparison to the whole cell. The analysis in terms of molecular function indicated that nuclear intrinsically disordered proteome (IDP-ome) is enriched in proteins involved in transcription regulation and especially in transcription factors.

INSTRUMENT(S): micrOTOF-Q II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Bozena Skupien-Rabian

LAB HEAD: Sylwia Kedracka-Krok

PROVIDER: PXD002676 | Pride | 2015-09-21

REPOSITORIES: Pride

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			Action	DRS
	N1.baf	Other
	N1.dat-pride.pride.mgf.gz	Mgf
	N1.dat-pride.pride.mztab.gz	Mztab
	N1.dat-pride.xml.gz	Xml
	N1_exclusion.baf	Other

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Proteomic and bioinformatic analysis of a nuclear intrinsically disordered proteome.

Skupien-Rabian Bozena B Jankowska Urszula U Swiderska Bianka B Lukasiewicz Sylwia S Ryszawy Damian D Dziedzicka-Wasylewska Marta M Kedracka-Krok Sylwia S

Journal of proteomics 20150912

<h4>Unlabelled</h4>Intrinsically disordered proteins (IDPs) are biologically active and crucial for cell function although they do not possess defined three-dimensional architecture. IDPs are especially prevalent in eukaryotic proteomes, and large-scale experiments have shown that many IDPs are nuclear proteins. Bioinformatic analyses have also demonstrated that the vast majority of transcription factors contain extended regions of intrinsic disorder. In the current study, we isolated and functi ...[more]

PMID: 26376097

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