Dataset Information

Proteomic analysis of cancer-related alterations in the human unfoldome

ABSTRACT: In this study, we explore the role of intrinsically disordered proteins (IDPs) in human breast cancer within the context of the "unfoldome." Using advanced proteomic techniques, we identified 2,271 protein groups in both normal and cancerous cell proteomes. Notably, 148 IDPs displayed significant differential expression in cancer cells, with a particular focus on cancer-related proteins. In our functional annotation, we found that 65% of these IDPs were linked to various diseases, with 20% specifically associated with cancer. Moreover, a substantial portion of the differentially expressed IDPs contained disordered regions, as verified through in silico characterization. By classifying IDPs into major Gene Ontology categories and conducting pathway analysis, our results highlight a high degree of interactivity within the altered portion of the human unfoldome in cancer, emphasizing the prevalence of moderately and highly disordered proteins in these networks. This study further elucidates the pivotal role of IDPs in the molecular mechanisms of breast cancer.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Lincoln Liburd

LAB HEAD: Dr. Siddharth Pratap

PROVIDER: PXD047284 | Pride | 2024-05-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	VP0517_Hum_MP_s-A_step-1.RAW.mzxml	Mzxml
	VP0517_Hum_MP_s-A_step-1.mgf	Mgf
	VP0517_Hum_MP_s-A_step-10.RAW.mzxml	Mzxml
	VP0517_Hum_MP_s-A_step-10.mgf	Mgf
	VP0517_Hum_MP_s-A_step-2.RAW.mzxml	Mzxml

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Publications

The Proteomic Analysis of Cancer-Related Alterations in the Human Unfoldome.

Paromov Victor V Uversky Vladimir N VN Cooley Ayorinde A Liburd Lincoln E LE Mukherjee Shyamali S Na Insung I Dayhoff Guy W GW Pratap Siddharth S

International journal of molecular sciences 20240126 3

Many proteins lack stable 3D structures. These intrinsically disordered proteins (IDPs) or hybrid proteins containing ordered domains with intrinsically disordered protein regions (IDPRs) often carry out regulatory functions related to molecular recognition and signal transduction. IDPs/IDPRs constitute a substantial portion of the human proteome and are termed "the unfoldome". Herein, we probe the human breast cancer unfoldome and investigate relations between IDPs and key disease genes and pat ...[more]

PMID: 38338831

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