Proteomics

Dataset Information

0

Hb Oxidation in stored RBCs and Vesicles


ABSTRACT: We used a state of the art proteomics workflow, based upon nano-UPLC-MS\MS and advanced database searching to identify oxidative modifications to functional residues of hemoglobin subunit beta . Progressive accumulation of oxidized residues in stored erythrocytes and selective removal in vesicles was observed, further substantiating the hypothesis about vesiculation as a self-protecting mechanism in aging erthrocytes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Blood

DISEASE(S): Disease Free

SUBMITTER: Matthew Wither  

LAB HEAD: Angelo D'Alessandro

PROVIDER: PXD002798 | Pride | 2015-10-05

REPOSITORIES: Pride

Dataset's files

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Publications

Hemoglobin oxidation at functional amino acid residues during routine storage of red blood cells.

Wither Matthew M   Dzieciatkowska Monika M   Nemkov Travis T   Strop Pavel P   D'Alessandro Angelo A   Hansen Kirk C KC  

Transfusion 20151001 2


<h4>Background</h4>Routine storage of red blood cells (RBCs) results in the progressive accumulation of storage lesions. While the clinical relevance of these lesions is still a matter of debate, alterations to RBC morphology and biochemistry, especially in terms of energy and redox homeostasis, are likely to affect RBC physiology and functionality at a minimum. Identification of oxidative modifications that accumulate on key RBC proteins will help bridge the gap between storage induced alterati  ...[more]

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